Screening of specific antigens for SARS clinical diagnosis using a protein microarray

Screening of specific antigens for SARS clinical diagnosis using a protein microarray

Source :

• Analyst [ 0003-2654 ] ; 2005.

Descripteurs français

• KwdFr :
  • Analyse par réseau de protéines, Anticorps antiviraux (analyse), Antigènes viraux (isolement et purification), Bioréacteurs, Escherichia coli, Humains, Immunoglobuline G (analyse), Protéines de fusion recombinantes (analyse), Protéines et peptides de signalisation intracellulaire (génétique), Protéines membranaires, Sensibilité et spécificité, Spectrométrie de masse MALDI, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Test ELISA (), Transporteurs d'anions organiques (génétique), Virus du SRAS (immunologie), Électrophorèse sur gel de polyacrylamide, Études cas-témoins.
• MESH :
  • analyse : Anticorps antiviraux, Immunoglobuline G, Protéines de fusion recombinantes.
  • diagnostic : Syndrome respiratoire aigu sévère.
  • génétique : Protéines et peptides de signalisation intracellulaire, Transporteurs d'anions organiques.
  • immunologie : Virus du SRAS.
  • isolement et purification : Antigènes viraux.
  • virologie : Syndrome respiratoire aigu sévère.
  • Analyse par réseau de protéines, Bioréacteurs, Escherichia coli, Humains, Protéines membranaires, Sensibilité et spécificité, Spectrométrie de masse MALDI, Test ELISA, Électrophorèse sur gel de polyacrylamide, Études cas-témoins.

English descriptors

• KwdEn :
  • Antibodies, Viral (analysis), Antigens, Viral (isolation & purification), Bioreactors, Case-Control Studies, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli, Humans, Immunoglobulin G (analysis), Intracellular Signaling Peptides and Proteins (genetics), Membrane Proteins, Organic Anion Transporters (genetics), Protein Array Analysis, Recombinant Fusion Proteins (analysis), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
• MESH :
  • chemical , analysis : Antibodies, Viral, Immunoglobulin G, Recombinant Fusion Proteins.
  • chemical , genetics : Intracellular Signaling Peptides and Proteins, Organic Anion Transporters.
  • chemical , isolation & purification : Antigens, Viral.
  • diagnosis : Severe Acute Respiratory Syndrome.
  • immunology : SARS Virus.
  • methods : Enzyme-Linked Immunosorbent Assay.
  • virology : Severe Acute Respiratory Syndrome.
• Teeft :
  • Affinity column, Amersham pharmacia, Amino acids, Antibody screening, Antibody testing, Antigen screening platform, Antigenic, Antisense primers, Assay, Beijing, Beijing hospital, Bioreactors, Calibration curve, Calibration curves, Case-Control Studies, Cell lysates, Clinical assessment, Clinical diagnosis, Clinical patient sera, Clinical trials, Coli, Coli dh5a, Convalescent patients, Coronavirus, Cutoff value, Detection limits, Detection wavelength, Different reactivities, Electrophoresis, Polyacrylamide Gel, Elisa, Elisa assay, Epidemiologic investigations, Escherichia coli, Experimental conditions, Expression plasmids, Fluorescence intensity, Fragment, Fusion protein, Fusion proteins, Gaattc, Gene fragments, Gene nucleotide sequence, Gene sequence, Ggatcc, Glass slides, Healthy individuals, High degree, Host cells, Human sera, Humans, Inpatient, Internal calibration curves, Lysates, Membrane Proteins, Microarray, Microarray assay, Microarrays, Molecular protein standards, Molecular weight, Negative control, Negative control sera, Negative sera, Novel coronavirus, Nucleocapsid proteins, Nucleotide, Nucleotide sequences, Oligonucleotide primers, Plasmid, Positive rates, Positive reactivity, Positive sera, Primer, Probable sars patients, Protein, Protein Array Analysis, Protein fragment screening microarray, Protein fragment screening microarrays, Protein fragments, Protein microarray, Protein microarray assay, Protein microarrays, Protein microarrays protein microarrays, Recombinant, Recombinant fusion protein, Recombinant proteins, Respiratory syndrome, Restriction sites, Royal society, Sars, Sars diagnosis, Sars disease, Sars infection, Sars inpatients, Sars outbreak, Sars patients, Sars sera, Scan images, Schematic representation, Screening, Screening microarrays, Sensitivity and Specificity, Sensitivity control sample, Serum samples, Sonication buffer, Specific antibodies, Specific antigens, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Structural proteins, Structure protein screening microarrays, Traditional chinese, Various structure proteins, Vector protein, Western medicine.

Abstract

• https://api.istex.fr/ark:/67375/QHD-7B8CRX67-C/fulltext.pdf

Affiliations:

• République populaire de Chine
• Pékin

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